RESEARCH ARTICLE
Nobel Med 2024; 20(2): 135-140

A CLASTOGENICITY STUDY WITH MITOMYCIN-C IN APLASTIC ANEMIA: DISTINGUISHING FANCONI ANEMIA

Gülçin Bağatir, Şükrü Öztürk, Ayşe Gül Bayrak Tokaç, Kıvanç Çefle, Aysegül Ünüvar, Zeynep Karakaş, Ayşe Palanduz, Şükrü Palanduz
ABSTRACT
Objective: Aplastic anemia is a rare condition characterized by bone marrow failure due to various etiologies including ffanconi anemia. Mitomycin C is an alkylating agent inducing chromosome breaks in aplastic anemia. The clastogenic effect of mitomycin C is most marked in ffanconi anemia. This study aims to demonstrate mitomycin C induced chromosomal breaks in patients with aplastic anemia and distinguish ffanconi anemia patients among them.

Material and Method: Clastogenicity test was applied to 147 patients with aplastic anemia, four siblings and 30 healthy controls. Both simultaneous and mitomycin C (MMC) treated lymphocyte cultures were prepared. Chromosome breaks per metaphase were calculated and cytogenetic abnormalities were analyzed.

Results: The mean number of chromosome breaks per metaphase increased from 0.02 to 0.37 after MMC treatment in patients with aplastic anemia. This number changed only from 0.01 to 0.16 in the healthy controls. In 25 patients (16.6%), the number of chromosome breaks per metaphase was ≥ 1 in MMC treated cultures, and MMC treatment induced a significant increase compared to spontaneous culture (2.88 vs 0.35), which assured the diagnosis of ffanconi anemia. We observed various multiple chromosomal aberrations in all of them and accompanying phenotypic features in 20.

Conclusion: Mitomycin C induced chromosomal breaks and cytogenetic abnormalities enable the diagnostic differentiation between aplastic anemia and ffanconi anemia.
Keywords
Aplastic anemia Ffanconi anemia Bone marrow failure Mitomycin C

Authors Orcid Profiles

Gülçin Bağatir
Şükrü Öztürk
Ayşe Gül Bayrak Tokaç
Kıvanç Çefle
Aysegül Ünüvar
Zeynep Karakaş
Ayşe Palanduz
Şükrü Palanduz

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  • Pubmed Style
    Gülçin Bağatir, Şükrü Öztürk, Ayşe Gül Bayrak Tokaç, Kıvanç Çefle, Aysegül Ünüvar, Zeynep Karakaş, Ayşe Palanduz, Şükrü Palanduz. [A CLASTOGENICITY STUDY WITH MITOMYCIN-C IN APLASTIC ANEMIA: DISTINGUISHING FANCONI ANEMIA]. Nobel Med 2024; 20(2): 135-140, English.
  • Web Style
    Gülçin Bağatir, Şükrü Öztürk, Ayşe Gül Bayrak Tokaç, Kıvanç Çefle, Aysegül Ünüvar, Zeynep Karakaş, Ayşe Palanduz, Şükrü Palanduz. [A CLASTOGENICITY STUDY WITH MITOMYCIN-C IN APLASTIC ANEMIA: DISTINGUISHING FANCONI ANEMIA]. www.nobelmedicus.com/en/Article.aspx?m=1716 [Access: Mayıs 24, 2021], English.
  • AMA (American Medical Association) Style
    Gülçin Bağatir, Şükrü Öztürk, Ayşe Gül Bayrak Tokaç, Kıvanç Çefle, Aysegül Ünüvar, Zeynep Karakaş, Ayşe Palanduz, Şükrü Palanduz. [A CLASTOGENICITY STUDY WITH MITOMYCIN-C IN APLASTIC ANEMIA: DISTINGUISHING FANCONI ANEMIA]. Nobel Med 2024; 20(2): 135-140, English.
  • Vancouver/ICMJE Style
    Gülçin Bağatir, Şükrü Öztürk, Ayşe Gül Bayrak Tokaç, Kıvanç Çefle, Aysegül Ünüvar, Zeynep Karakaş, Ayşe Palanduz, Şükrü Palanduz. [A CLASTOGENICITY STUDY WITH MITOMYCIN-C IN APLASTIC ANEMIA: DISTINGUISHING FANCONI ANEMIA]. Nobel Med (2024); 20(2): 135-140, [cited Mayıs 24, 2021], English.
  • Harvard Style
    Gülçin Bağatir, Şükrü Öztürk, Ayşe Gül Bayrak Tokaç, Kıvanç Çefle, Aysegül Ünüvar, Zeynep Karakaş, Ayşe Palanduz, Şükrü Palanduz. (2024) [A CLASTOGENICITY STUDY WITH MITOMYCIN-C IN APLASTIC ANEMIA: DISTINGUISHING FANCONI ANEMIA]. Nobel Med, 20(2): 135-140, English.